Clubroot (Plasmodiophora brassicae) are a rich source of glucosinolates, and it includes glucoraphanin which produces the isothiocyanate sulforaphane which has possessed chemo-protective activity in humans. It defends plant against herbivoreand pathogen attack.Clubroot, caused by Plasmodiophora brassicae, is an important and common disease worldwide however the effect of clubroot on glucosinolate levels is unclear. The combination of incorporation of infected gall slurry into potting mix before planting was found to cause faster and more consistent clubroot infection significantly affected glucosinolate levels in disease development than single inoculation methods. Both field and glasshouse grown brassicas are affected by Clubroot infection. . In a glasshouse trial, P. brassicae affected glucosinolate levels in both root and aerial tissues of broccoli seedlings during primary, secondary and mature gall formation. Aliphatic glucosinolate levels (glucoiberin, progoitrin, glucoraphanin, gluconapin) remained constant in aerial tissues but significantly increased in root tissues during 28 days post inoculation.  Due to this infection progoitrin levels in leaf and stem tissues decreased but significantly increased in roots. Indole glucosinolate levels also increased significantly. The results indicate that clubroot infection affect glucosinate classes differently in varying plant tissues.

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