With the rapid growth in population, Plant biotechnology has become a must in ensuring food security which could hardly be met by traditional agriculture. In vitro Plant cell cultures have been used since a considerable long time for molecular farming and the products are already being commercialized. Therefore, it is of utmost concern to preserve the plant germplasm,for which Cryopreservation could be considered as an ideal technique. Maintaining of transgenic materials by repeated subculture is time consuming, labor cost, has got chances of culture loss due to contamination and most of all the risk of transgene loss or gene flow. When these demerits are taken into account, cryopreservation is the best possible way for preserving transgenic materials. Till date there have been many studies done attempting cryopreserving transgenic materials and all the results obtained from these studies indicated that the technique not only does no hamper to the expression of foreign genes in the transgenic material but also conserves the productive ability of the cells containing recombinant proteins.
After the first report on successful cryopreservation of plants published in 1960, various cryopreservation techniques such as droplet, droplet-vitrification, vitrification, encapsulation-dehydration and encapsulation-vitrification have been successfully developed for economically important crops such as woody and herbaceous plants ranging from tropical to temperate regions. Although the main limitation i.e. strong genotypic response is involved in Cryopreservation, it has been widely accepted as an efficient way of preservation as it arrests the cellular divisions and metabolic processes ensuring genetic stability.Tags: Cryopreservation, Plant Biotechnology, Transgenic Materials